ABP Biosciences提供3种慢病毒转染增强剂。
1. Lentivirus Transduction Enhancer I
Lentivirus Transduction Enhancer I is a novel chemical reagent that increases lentiviral transduction efficiency into in vitro systems. Normally, incubation of lentivirus particles in cells in the presence of polybrene is generally efficient in transducing many cell types. However, lentiviruses are typically attenuated by cellular antiviral defense mechanism, which limits their transducing efficiency. Lentivirus Transduction Enhancer I suppresses the cellular anti-viral state by mimicking the activity of viral virulence gene products, and thus significantly increases lentivirus transducing efficiency by as high as 10-fold.
2. Lentivirus Transduction Enhancer II
Lentivirus Transduction Enhancer II is a nontoxic novel chemical reagent that increases lentiviral transduction efficiency by modulating membrane permeability. Normally, incubation of lentivirus particles in cells in the presence of polybrene is generally efficient in transducing many cell types. However, due to polycation property, polybrene is toxic for some sensitive cells. Lentivirus Transduction Enhancer II is a neutral and nontoxic transduction enhancer, which significantly increases lentivirus transducing efficiency while maintaining cell membrane integrity.
3. Polybrene
As a cationic polymer, polybrene increases the efficiency of infection in some cells by neutralizing the charge repulsion between virions and the cell surface.
Polybrene is most effective when added to cell culture media at the time of transduction. Recommended working concentration is 1:100. However, the working concentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimize the effect.