LipoFectMax™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. LipoFectMax™ Transfection Reagent has been tested to work the same efficiency as Lipofectamine® 2000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.
Features
- Superior transfection efficiency for a broad range of cell lines
- Does not require removal of serum or culture medium
- Does not require washing or changing of medium after transfection
- Low cytotoxicity
图1. LipoFectMax™转染试剂以绿色荧光蛋白（GFP）表达质粒转染几种常见细胞系，通过检测GFP信号比较转染效率。
图2. LipoFectMax™, Lipofectamine® 2000及Lipofectamine® 3000分别对A549细胞进行转染操作，通过计算转染后的细胞存活率比较细胞毒性。
图3. 用LipoFectMax™转染Hela细胞, 左图转染GFP cDNA, 右图共转染GFP cDNA和GFP靶向siRNA 。转染siRNA后可以从右图明显看到基因沉默。
图4. 用LipoFectMax™和 Lipofectamine® 2000分别对小鼠神经元细胞进行转染绿色荧光蛋白（GFP），转染24小时后观察转染效果， LipoFectMax™转染效率（左图）比Lipofectamine® 2000（右图）高5倍。