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LipoFectMax™

Introduction

LipoFectMax™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. LipoFectMax™ Transfection Reagent has been tested to work the same efficiency as Lipofectamine® 2000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.

Features

  • Superior transfection efficiency for a broad range of cell lines
  • Does not require removal of serum or culture medium
  • Does not require washing or changing of medium after transfection
  • Low cytotoxicity


图1. LipoFectMax™转染试剂以绿色荧光蛋白(GFP)表达质粒转染几种常见细胞系,通过检测GFP信号比较转染效率。


图2. LipoFectMax™, Lipofectamine® 2000及Lipofectamine® 3000分别对A549细胞进行转染操作,通过计算转染后的细胞存活率比较细胞毒性。


图3. 用LipoFectMax™转染Hela细胞, 左图转染GFP cDNA, 右图共转染GFP cDNA和GFP靶向siRNA 。转染siRNA后可以从右图明显看到基因沉默。


图4. 用LipoFectMax™和 Lipofectamine® 2000分别对小鼠神经元细胞进行转染绿色荧光蛋白(GFP),转染24小时后观察转染效果, LipoFectMax™转染效率(左图)比Lipofectamine® 2000(右图)高5倍。

To Order

Buy Cat.No. Product Name Unit Price
FP310 LipoFectMax™ Transfection Reagent 1 mL 2000
FP311 LipoFectMax™ Transfection Reagent 3 mL 5000

Documents

Protocol (PDF): FP310
MSDS (PDF):  MSDS-FP310
MSDS-FP311