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LipoFectMax™

Introduction

LipoFectMax™ Transfection Reagent is a lipid-based transfection reagent that forms a complex with DNA or RNA, and transports the complex into a variety of adherent and suspension cell lines. LipoFectMax™ Transfection Reagent has been tested to work the same efficiency as Lipofectamine® 2000 Reagent, and used for the transfection of both DNA and RNA into eukaryotic cells even in the presence of serum.

Features

  • Superior transfection efficiency for a broad range of cell lines
  • Does not require removal of serum or culture medium
  • Does not require washing or changing of medium after transfection
  • Low cytotoxicity


图1. LipoFectMax™转染试剂以绿色荧光蛋白(GFP)表达质粒转染几种常见细胞系,通过检测GFP信号比较转染效率。


图2. LipoFectMax™, Lipofectamine® 2000及Lipofectamine® 3000分别对A549细胞进行转染操作,通过计算转染后的细胞存活率比较细胞毒性。


图3. 用LipoFectMax™转染Hela细胞, 左图转染GFP cDNA, 右图共转染GFP cDNA和GFP靶向siRNA 。转染siRNA后可以从右图明显看到基因沉默。


图4. 用LipoFectMax™和 Lipofectamine® 2000分别对小鼠神经元细胞进行转染绿色荧光蛋白(GFP),转染24小时后观察转染效果, LipoFectMax™转染效率(左图)比Lipofectamine® 2000(右图)高5倍。

To Order

Buy Cat.No. Product Name Unit Price
FP310 LipoFectMax™ Transfection Reagent 1 mL 2000
FP311 LipoFectMax™ Transfection Reagent 3 mL 5000

Documents

 

Protocol (PDF): FP310
MSDS (PDF):  MSDS-FP310
MSDS-FP311

 

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Generation of C-to-G transversion in mouse embryos via CG editors
Cao, T., Liu, S., Qiu, Y. et al.
Transgenic Res 31, 445–455 (2022). https://doi.org/10.1007/s11248-022-00313-x


RIPK3 Promotes JEV Replication in Neurons via Downregulation of IFI44L
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Front. Microbiol. 2020, 11:368. doi: 10.3389/fmicb.2020.00368


Stanniocalcin 2 Is Upregulated by Calcium-Sensing Receptor and Protects Human Vascular Smooth Muscle Cells from High-Phosphate-Induced Apoptosis
Liu F, Zhang A, Huang H, Liu X, Diao Z, Deng D, Liu W
Cardiorenal Med 2022;12:214-228. doi: 10.1159/000527441


STC2: A Novel and Regulable Protector of Vascular Smooth Muscle Cells
Zhang, Ai-hua and Liu, Fen-fen and Huang, Hong-dong and Liu, Xu and Diao, Zong-li and Deng, Dai and Liu, Wen-hu
http://dx.doi.org/10.2139/ssrn.4010831


Axl-/- neurons promote JEV infection by dampening the innate immunity
Jiali Yang, Mengyuan Li, Mingcheng Yuan, Peiyu Bian, Yangchao Dong, Haijun Zhang, Chuanyu Luo, Zhifeng Xue, Yuan Wang, Fanglin Zhang, Lixin Shen, Yingfeng Lei
Virus Research, 2022, 307, 198605, https://doi.org/10.1016/j.virusres.2021.198605


Endothelial and systemic upregulation of miR-34a-5p fine-tunes senescence in progeria.
Manakanatas C, Ghadge SK, Agic A, Sarigol F, Fichtinger P, Fischer I, Foisner R, Osmanagic-Myers S.
Aging (Albany NY). 2022 Jan 12;14(1):195-224. doi: 10.18632/aging.203820


A novel homozygous RIPK4 variant in a family with severe Bartsocas-Papas syndrome
Dinçer, T., Gümüş, E., Toraman, B., Er, Yildiz, G., Yüksel, Z., & Kalay, E.
Am J Med Genet. 2021;185A:1691–1699. doi:10.1002/ajmg.a.62154


Comprehensive Pan-Cancer Analyses of Immunogenic Cell Death as a Biomarker in Predicting Prognosis and Therapeutic Response.
Wang, Y.; Huang, Y.; Yang, M.; Yu, Y.; Chen, X.; Ma, L.; Xiao, L.; Liu, C.; Liu, B.; Yuan, X.
Cancers 2022, 14, 5952. https://doi.org/10.3390/cancers14235952


Hydroxychloroquine impairs mTORC1-dependent cholesterol biosynthesis and sensitizes retinal pigment epithelial cells to UV-induced cell death
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Research Square, 2022, https://doi.org/10.21203/rs.3.rs-2299218/v1


PTPRH Alleviates Airway Obstruction and Th2 Inflammation in Asthma as a Protective Factor
Feng-jia Chen, Li-juan Du, Zhimin Zeng, Xin-yan Huang, Chang-yi Xu, Wei-ping Tan, Can-mao Xie, Yu-xia Liang & Yu-biao Guo
Journal of Asthma and Allergy, 2022, 15: 133-144. DOI: 10.2147/JAA.S340059


Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells
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Comprehensive Pan-Cancer Analyses of Immunogenic Cell Death as a Biomarker in Predicting Prognosis and Therapeutic Response.
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