PolyFect™ Transfection Reagent

PolyFect™ Transfection Reagent is a biodegradable polymer based transfection reagent that forms a complex with DNA, and transports the complex into a variety of adherent and suspension cell lines. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis, leading to much less cytotoxicity.

Features

• Superior transfection efficiency for a broad range of cell lines
• Does not require removal of serum or culture medium
• Does not require washing or changing of medium after transfection
• Low cytotoxicity

Figure 1. Comparison of PolyFect™ and Lipofectamine® 2000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using either PolyFect™ transfection reagent (yellow), or Lipofectamine® 2000 (blue). Cells were evaluated for transfection efficiencies after 48 hours post-transfection.

Figure 2. The transfection efficiencies of PolyFect™ in Expi293F, and CHO suspension cells are over 95%, ideal for protein expression.