Applied BioProbes has developed a breakthrough antibody labeling technology with Andy Fluor™ dyes — iLink™ Antibody Labeling Kits. With this technology, you only need to mix your antibody with Andy Fluor™ dye provided in the kit, and in 30 minutes you will have an optimally labeled Andy Fluor™ dye-antibody conjugate ready for immunostaining. The labeling technology provides unprecedented convenience for multicolor immunostaining, where pre-labeled primary antibodies may not be available, and indirect staining via pre-labeled fluorescent secondary antibodies may also be difficult due to cross-interaction among different antibodies.
Features
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iLink™ Antibody Labeling Kit Selection Guide
Fluorophore | Ex/Em (nm) | Imaging | Flow cytometry | Cat. No. |
Biotin | – | ✔ | L030 | |
FITC | 495/518 | ✔ | ✔ | L031 |
Andy Fluor™ 350 | 350/440 | ✔ | L032 | |
Andy Fluor™ 405M | 405/450 | ✔ | ✔ | L033 |
Andy Fluor™ 488 | 495/520 | ✔ | ✔ | L034 |
Andy Fluor™ 555 | 553/565 | ✔ | L035 | |
Andy Fluor™ 568 | 578/602 | ✔ | L036 | |
Andy Fluor™ 594 | 590/615 | ✔ | L037 | |
Andy Fluor™ 647 | 650/666 | ✔ | ✔ | L038 |
How iLink™ Antibody Labeling Kits work
The Antibody Labeling Kits utilize an amine-reactive Andy Fluor™ fluorophore to covalently attach the label to the IgG antibody of interest. The Antibody Labeling Kits are optimized for labeling 50-100 µg of antibody per reaction, and contain everything you need to perform the conjugation. Simply mix your antibody with a vial of pre-measured Andy Fluor™ dye, biotin, or FITC, and wait for 30 minutes, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.
Figure 1. Label your antibody in 30 minutes.
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