Applied BioProbes offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable Andy Fluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.
Advantages
- Bright signals with low background
- Wide variety of fluorophores.
- Great for many applications, like Fluorescence in situ Hybridization (FISH; Figure 1), TUNEL (Figure 2), and more.
Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.
Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).
Labeled nucleotides
Related Products
- Nucleic Acid Gel Stains
- Nucleic Acid Quantitation
- Labeled Nucleotides
- Protein Detection and Quantitation
- Cell Structure Probes
- Secondary Antibody and Streptavidin
- Cell Proliferation & Viability Assay
- Cell Apoptosis Assay
- Andy Fluor™ Dyes
- Antibody Labeling Kits
- Click Chemistry
- Ion Indicators
- Enzyme Substrates
- Reactive Dyes
- Biotin Derivatives
- ROS Probes