TRIzol Reagent是均相溶液，用来快速裂解细胞/组织，释放RNA至溶液中，并可快速灭活核酸酶，保护RNA不降解。经过氯仿抽提后形成三相体系，其中RNA分布于水相层，DNA 和蛋白质分布于中间层和有机层，达到分离RNA 的目的。TRIzol Reagent适合于从各种生物样品中快速提取高纯度的总RNA。纯化的RNA 可直接用于RT-PCR、Northern 杂交、Poly(A)富集等下游应用。此外，TRIzol Reagent还可以用于DNA和蛋白质的提取。
Features
- Permits the isolation of RNA, DNA, and protein from the same sample
- Offers superior lysis capability, even with difficult sample types
- Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
| Protocol (PDF): | FP312 |
| MSDS (PDF): | MSDS-FP312 |
Cited Reference:
Identification of Diagnostic Markers Correlated With HIV+ Immune Non-response Based on Bioinformatics Analysis
Bai R, Li Z, Hou Y, Lv S, Wang R, Hua W, Wu H and Dai L
Front. Mol. Biosci. 2021, 8:809085. doi: 10.3389/fmolb.2021.809085
Identification and Validation of Hub Genes Associated with Hepatocellular Carcinoma Via Integrated Bioinformatics Analysis
Wang S, Song Z, Tan B, Zhang J, Zhang J and Liu S
Front Oncol (2020) 10:681. doi: 10.3389/fonc.2020.00681
Exploring the biological function of immune cell-related genes in human immunodeficiency virus (HIV)-1 infection based on weighted gene co-expression network analysis (WGCNA)
Bai, R., Li, Z., Lv, S. et al.
BMC Med Genomics 15, 200 (2022). https://doi.org/10.1186/s12920-022-01357-y
Cell Proliferation and Tumor Induction by Ochratoxin A in Mouse Skin and Evaluation of Cyclin D1 and Cyclooxygenase-2 Expressions
Sai Yang and Yajia Wen
Computational and Mathematical Methods in Medicine, 2021, Article ID 1723486, https://doi.org/10.1155/2021/1723486
Differentially Infiltrated Identification of Novel Diagnostic Biomarkers Associated with Immune Infiltration in Nasopharyngeal Carcinoma
Pei Gao , Wuhao Lu, Shousen Hu, and Kun Zhao
Disease Markers, 2022, Article ID 3934704, https://doi.org/10.1155/2022/3934704
Screening of DNA Damage Repair Genes Involved in the Prognosis of Triple-Negative Breast Cancer Patients Based on Bioinformatics
Wang N, Gu Y, Chi J, Liu X, Xiong Y, Zhong C, Wang F, Wang X and Li L
Front. Genet. 2021, 12:721873. doi: 10.3389/fgene.2021.721873
Identification and validation of HOXD3 and UNC5C as molecular signatures in keloid based on weighted gene co-expression network analysis
Hanwen Wang, Ziheng Zhou, Yiling Liu, Peng Wang, Lei Chen, Shaohai Qi, Julin Xie, Jinming Tang
Genomics, 2022, 114, 4, 110403, https://doi.org/10.1016/j.ygeno.2022.110403
A Coagulation-Related Gene-Based Prognostic Model for Invasive Ductal Carcinoma
Li J, Du J, Wang Y and Jia H
Front. Genet. 2021, 12:722992. doi: 10.3389/fgene.2021.722992
Identification of CALU and PALLD as Potential Biomarkers Associated with Immune Infiltration in Heart Failure.
Liu X, Xu S, Li Y, Chen Q, Zhang Y and Peng L
Front. Cardiovasc. Med. 2021, 8:774755. doi: 10.3389/fcvm.2021.774755
Comparative bioinformatics analysis and abiotic stress responses of expansin proteins in Cucurbitaceae members: watermelon and melon
Incili, Ç.Y., Arslan, B., Çelik, E.N.Y. et al.
Protoplasma (2022). https://doi.org/10.1007/s00709-022-01793-8
Dexmedetomidine Mitigates Myocardial Ischemia/Reperfusion-Induced Mitochondrial Apoptosis through Targeting lncRNA HCP5
Xu Deng, Fei Ye, Lixiong Zeng, Wenzhi Luo, Shan Tu, Xiaoyan Wang and Zhihui Zhang
The American Journal of Chinese Medicine, 2022, 50, 1529-1551. https://doi.org/10.1142/S0192415X22500641
Integrated analysis of differential lncRNA and mRNA expression in cholelithiasis based on high-throughput sequencing and bioinformatics
C Xu, Y Sun, Y Li, Q Xu, W Li, P Xu
Research Square, 2022. DOI: https://doi.org/10.21203/rs.3.rs-1708027/v1
Glycosyltransferases EXTL2 and EXTL3 cellular balance dictates heparan sulfate biosynthesis and shapes gastric cancer cell motility and invasion
Catarina Marques, Juliana Poças, Catarina Gomes, Isabel Faria-Ramos, Celso A. Reis, Romain R. Vivès, and Ana Magalhães
J. Biol. Chem. (2022) 298(11) 102546. https://doi.org/10.1016/j.jbc.2022.102546
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