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Tyramide Signal Amplification (TSA)


Tyramide signal amplification (TSA) is a highly sensitive method enabling the detection of low-abundance targets in fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (FISH) applications.

Tyramide signal amplification (TSA) process involves the use of horseradish peroxidase (HRP) to catalyze the deposition of fluorophore tyramides on and near a target protein or nucleic acid sequence in situ. In the presence of low concentrations of H2O2, HRP is able to convert a labeled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on proteins at or near the HRP. This generates high density tyramide labeling and is the reason for the exceptional sensitivity of this system. 


  • Highly sensitive detection of low-abundance, hard-to-detect targets
  • Compatible with ICC, IHC and FISH
  • High-resolution and multiplex imaging
  • Similar workflow to conventional ICC, IHC and FISH

Figure 1. Illustration of the tyramide signal amplification system.

Figure 2. Multiplex tyramide labeling of FFPE tissues. 


To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
L040 Alexa Fluor 488 Tyramide 505/526 0.5 µmol 2100
L041 Alexa Fluor 555 Tyramide 553/565 0.5 µmol 2100
L042 Alexa Fluor 594 Tyramide 590/615 0.5 µmol 2100
L043 Alexa Fluor 647 Tyramide 650/666 0.5 µmol 2100