Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The 5-FAM azide is reactive with terminal alkyne via a copper-catalyzed click reaction that allows the subsequent visualization by fluorescence spectroscopy.
Features
- Efficiency—the click reaction is complete in less than 1 hour;
- Specificity—the reaction between the label and detection tag is selective and specific;
- Stability—the reaction product contains an irreversible, covalent bond;
- Biologically inert—the components of the reaction do not undergo any side reactions.
Figure 1. Click chemistry labeling
Specifications
Label: | FAM | |
Ex/Em: | 495/518 | |
Detection Method: | Fluorescent | |
Solubility: | DMSO, DMF | |
Molecular Weight: | 532.51 | |
Product Size: | 1 µmol | |
Storage Conditions: | -20 ℃, protect from light | |
Shipping Condition: | Room Temperature | |
Applications Click chemistry labeling |
Datasheet (PDF): | C306 |
MSDS (PDF): | C306 |
Reference
Angew Chem Int Ed Engl (2012) 51:3143-3146
Nucleic Acids Res (2012) 40:e78-e78
Proc Natl Acad Sci U S A (2013) 110:4992-4997
Proc Natl Acad Sci U S A (2007) 104:2614-2619
Angew Chem Int Ed Engl (2009) 48:4030-4033
Related Products
- Biotin Alkyne
- Biotin Azide
- 5-FAM Alkyne
- 5-FAM Azide
- 5-TAMRA Alkyne
- 5-TAMRA Azide
- Texas Red Alkyne
- Texas Red Azide
- Cy3 Alkyne
- Cy3 Azide
- Cy5 Alkyne
- Cy5 Azide
- Andy Fluor 350 Alkyne
- Andy Fluor 350 Azide
- Andy Fluor 488 Alkyne
- Andy Fluor 488 Azide
- Andy Fluor 555 Alkyne
- Andy Fluor 555 Azide
- Andy Fluor 594 Alkyne
- Andy Fluor 594 Azide
- Andy Fluor 647 Alkyne
- Andy Fluor 647 Azide
- Biotin Picolyl Azide
- Andy Fluor 488 Picolyl Azide
- Andy Fluor 555 Picolyl Azide
- Andy Fluor 594 Picolyl Azide
- Andy Fluor 647 Picolyl Azide
- Cy3 Picolyl Azide
- Cy5 Picolyl Azide
- Biotin DBCO
- Andy Fluor 488 DBCO
- Andy Fluor 555 DBCO
- Andy Fluor 594 DBCO
- Andy Fluor 647 DBCO
- Cy3 DBCO
- Cy5 DBCO