Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
The Andy Fluor™ 555 DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC).
Features
- Efficiency—the click reaction is complete in less than 1 hour;
- Specificity—the reaction between the label and detection tag is selective and specific;
- Stability—the reaction product contains an irreversible, covalent bond;
- Biologically inert—the components of the reaction do not undergo any side reactions.
Figure 1. Click chemistry labeling
Specifications
Label: | Andy Fluor™ 555 | |
Ex/Em: | 553/565 | |
Detection Method: | Fluorescent | |
Solubility: | DMSO, DMF | |
Product Size: | 0.5 µmol | |
Storage Conditions: | -20 ℃, protect from light | |
Shipping Condition: | Room Temperature | |
Applications Click chemistry labeling |
Datasheet (PDF): | C332 |
MSDS (PDF): | C332 |
Reference
Angew Chem Int Ed Engl (2012) 51:3143-3146
Nucleic Acids Res (2012) 40:e78-e78
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