The BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure total protein concentration compared to a protein standard. The BCA Protein Assay Kit is based on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+ by cysteine, cystine, tryptophan, tyrosine, and peptide bonds. BCA forms a purple colored complex with Cu1+ that is detectable at 562nm, and proportional to the protein present. The BCA Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
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| Protocol (PDF): | P011 |
| MSDS (PDF): | MSDS-P011 |
| COA (PDF): | COA-P011 |
Cited Reference:
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Food Bioscience, 2023, 52, 102371. https://doi.org/10.1016/j.fbio.2023.102371
Acetylbritannilactone attenuates contrast-induced acute kidney injury through its anti-pyroptosis effects.
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Fusobacterium nucleatum Causes Microbial Dysbiosis and Exacerbates Visceral Hypersensitivity in a Colonization-Independent Manner
Gu X, Song L, Li L, Liu T, Zhang M, Li Z, Wang P, Li M and Zuo X
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TRIM31 enhances chemoresistance in glioblastoma through activation of the PI3K/Akt signaling pathway
Fan MD, Zhao XY, Qi JN, Jiang Y, Liu BY, Dun ZP, Zhang R, Wang CW and Pang Q
Exp Ther Med 20: 802-809, 2020. https://doi.org/10.3892/etm.2020.8782
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Jin B-Y, Li Z, Xia Y-N, Li L-X, Zhao Z-X, Li X-Y, Li Y, Li B, Zhou R-C, Fu S-C, Li S-Y and Li Y-Q
Front. Immunol. 2021, 12:741371. doi: 10.3389/fimmu.2021.741371
Tilapia skin peptides, a by-product of fish processing, ameliorate DSS-induced colitis by regulating inflammation and inhibiting apoptosis
Gao J, Li L, Zhao D, Wang X, Xia Y, Li B, Liu C and Zuo X
Front. Nutr. 2022, 9:988758. doi: 10.3389/fnut.2022.988758
Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis
Bing Li, Yan Li, Lixiang Li, Yu Yu, Xiang Gu, Chang Liu, Xin Long, Yanbo Yu & Xiuli Zuo
EPIGENOMICS, 2021, 13, 17. https://doi.org/10.2217/epi-2021-0230
Chemical characterization, gastrointestinal motility and sensory evaluation of dark chocolate: A nutraceutical boosting consumers' health
Caponio, G.R.; Lorusso, M.P.; Sorrenti, G.T.; Marcotrigiano, V.; Difonzo, G.; De Angelis, E.; Guagnano, R.; Ciaula, A.D.; Diella, G.; Logrieco, A.F.; Montagna, M.T.; Monaci, L.; De Angelis, M.; Portincasa, P.
Nutrients 2020, 12(4), 939; https://doi.org/10.3390/nu12040939
Antimicrobial and Anticancer Activity of Corydalis solida
Dulger G.
Saglık Bilimlerinde Deger. 2022; 12(3): 534-539. https://doi.org/10.33631/sabd.1145694