Biotin Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Biotin. Biotin label is used to subsequent detection by streptavidin for signal amplification.
Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
|Host/Isotype:||Goat||Physical State:||Lyophilized power|
|Type:||Secondary Antibody||Purification:||Immunoaffinity chromatography|
|Tested Species Reactivity:||Rabbit (Rb)||Buffer:||PBS, pH 7.4, with 0.1% BSA|
|Target Class:||IgG (H+L)||Preservative:||0.02% sodium azide|
|Antibody Form:||Whole Antibody||Storage Conditions:||4ºC; store in dark|
Immunofluorescent analysis of α-tubulin with Biotin Goat Anti-Rabbit IgG (H+L) Antibody
|Unit Size:||100 µg, 500 µg|
|Western Blot (WB)||1:5,000 – 1:20,000|
|Immunofluorescence (IF)||1:100 – 1:2,000|
|Immunocytochemistry (ICC)||1:100 – 1:2,000|
|Immunohistochemistry (IHC)||1:100 – 1:2,000|
|Flow Cytometry (FACS)||1:100 – 1:1,000|