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FITC (Fluorescein-5-isothiocyanate)

Introduction

FITC is a amine-reactive fluorescent probe for labeling of proteins. This amine-reactive fluorescein derivative has been the most common fluorescent derivatization reagent for covalently labeling of proteins.

Specifications

Label: Fluorescein
Ex/Em: 495/518 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C21H11NO5S
Molecular Weight: 389.4
CAS Number: 3326-32-7
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Protein labeling

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C100 FITC 495/518 50 mg $35
C101 FITC 495/518 500 mg $100

Documents

Datasheet (PDF): FITC
MSDS (PDF): MSDS-C100, MSDS-C101

References:

Conjugation of antibodies with fluorochrome: modifications to the standard methods
Goding, J.W.
J Immunol Meth (1976) 13:215-226
 
Fluorescein labeling of Fab while preserving single thiolFluorescein labeling of Fab while preserving single thiol
Der-Balian, G.P., et al.
Anal Biochem (1988) 173:59-63
 
Fluorescence detection in automated DNA sequence analysisFluorescence detection in automated DNA sequence analysis
Smith, L.M., et al.
Nature (1986) 321:674-678