Introduction
FITC is a amine-reactive fluorescent probe for labeling of proteins. This amine-reactive fluorescein derivative has been the most common fluorescent derivatization reagent for covalently labeling of proteins.
Specifications
Label:
|
Fluorescein
|
|
Ex/Em:
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495/518 nm
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Detection Method:
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Fluorescent
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Solubility:
|
DMSO, DMF
|
Molecular Formula:
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C21H11NO5S
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Molecular Weight:
|
389.4
|
CAS Number:
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3326-32-7
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Storage Conditions:
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-20 ℃, protect from light
|
Shipping Condition:
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Room Temperature
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Applications
Protein labeling
|
Documents
References:
Conjugation of antibodies with fluorochrome: modifications to the standard methods
Goding, J.W.
J Immunol Meth (1976) 13:215-226
Fluorescein labeling of Fab while preserving single thiolFluorescein labeling of Fab while preserving single thiol
Der-Balian, G.P., et al.
Anal Biochem (1988) 173:59-63
Fluorescence detection in automated DNA sequence analysisFluorescence detection in automated DNA sequence analysis
Smith, L.M., et al.
Nature (1986) 321:674-678