FITC Goat Anti-Mouse IgG (H+L) Antibody is prepared by labeling high quality goat anti-mouse IgG (H+L) with FITC. FITC is the traditional green fluorescent dye, which is easily suspected to photo-bleaching.
Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
| Specifications | Form Information | |||||
| Host/Isotype: | Goat | Physical State: | Lyophilized power | |||
| Class: | Polyclonal | Shipping Condition: | Ambient | |||
| Type: | Secondary Antibody | Purification: | Immunoaffinity chromatography | |||
| Tested Species Reactivity: | Mouse (Ms) | Buffer: | PBS, pH 7.4, with 0.1% BSA | |||
| Target Class: | IgG (H+L) | Preservative: | 0.02% sodium azide | |||
| Antibody Form: | Whole Antibody | Storage Conditions: | 4ºC; store in dark | |||
| Label: | FITC | |||||
| Excitation/Emission: | 495/518 nm |
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Immunofluorescent analysis of α-tubulin with FITC Goat Anti-Mouse IgG (H+L) Antibody |
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| Unit Size: | 100 µg, 500 µg | |||||
| Applications | Dilution | |||||
| Western Blot (WB) | 1:5,000 – 1:20,000 | |||||
| Immunofluorescence (IF) | 1:100 – 1:2,000 | |||||
| Immunocytochemistry (ICC) | 1:100 – 1:2,000 | |||||
| Immunohistochemistry (IHC) | 1:100 – 1:2,000 | |||||
| Flow Cytometry (FACS) | 1:100 – 1:1,000 | |||||
