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5-FAM-dUTP, 1 mM solution

Introduction

5-FAM-dUTP (cat. no. C405A) is supplied as a 1 mM solution in TE buffer. 5-FAM-dUTP is also available in lyophilized form (cat. no. C405B).

5-FAM-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.

Specifications:

Excitation/Emission (nm): 495/518 nm         
Concentration: 1 mM solution in TE buffer
Storage: Store at -20°C
Molecular Formula: C33H27N3Na3O20P3
Molecular Weight: 947.47

 

 

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

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To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C405A 5-FAM-dUTP, 1 mM in TE buffer 495/518 25 µL $140


Protocol

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Documents

Protocol (PDF): C405A
MSDS (PDF):  MSDS-C405A

Frequently asked questions (FAQs)

Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?
You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.

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