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蛋白凝胶电泳及相关的Western-Blot检测是蛋白分析的主流技术之一 (Figure 1)。ABP Biosciences 提供两类荧光蛋白染料: 1) eLuminol™ Protein Gel Stain,一种具有极高灵敏度的蛋白荧光染料,用于检测SDS-PAGE凝胶电泳中的总蛋白; 2) Phos-Tag™ Phosphoprotein Gel Stain,一种专门用于检测SDS-PAGE凝胶电泳中磷酸化蛋白的荧光染料。另外,ABP Biosciences 还提供BCA蛋白定量检测试剂盒。

Figure 1. Protein gel analysis workflow


  eLuminol™ Protein Gel Stain Phos-Tag™ Phosphoprotein Gel Stain
灵敏度 0.5 ng 1 ng
操作性 Supplied as stock solution Ready to use
耗时 90 min (standard)
30 min (microwave)
4 hr (standard)
2 hr (microwave)
检测方法 fluorescent fluorescent
质谱兼容 Yes Yes



Figure 2. Gel stain results with eLuminol™ Protein Gel Stain (left), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.


Figure 3. BSA standard curve with BCA Protein Assay Kit.


To order

Buy Catalog # Product Name Description Size List Price

eLuminol™ Protein Gel Stain (1,000X) 总蛋白染料 500 µL
1 mL
P005A Phos-Tag™ Phosphoprotein Gel Stain 磷酸化蛋白染料 500 mL 1200
P005B Phos-Tag™ Phosphoprotein Destain Solution 磷酸化蛋白洗脱液 500 mL 300
P011 BCA Protein Assay Kit BCA蛋白定量 1000 assays 500
P013 SDS-PAGE Sample Loading Buffer (5X) 上样缓冲液 5 mL 70