5-Ethynyl-dUTP (5-EdUTP) (cat. no. C432) is supplied as a 1 mM solution in TE buffer.
5-EdUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I.
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice
- to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
-
to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
|
Specifications:
Concentration: 1 mM solution in TE buffer
|
|
Applications
Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension.
Related Products
- 5-Aminoallyl-dUTP, 10 mM in TE buffer
- 5-Aminoallyl-UTP, 10 mM in TE buffer
- Biotin-11-dUTP, 1 mM in TE buffer
- Biotin-11-UTP, 10 mM in TE buffer
- 5-FAM-dUTP, 1 mM in TE buffer
- 5-FAM-X-dUTP, 1 mM in TE buffer
- 5-FAM-UTP, 1 mM in TE buffer
- 5-FAM-X-UTP, 1 mM in TE buffer
- Andy Fluor 488-X-dUTP, 1 mM in TE buffer
- Andy Fluor 488-X-UTP, 1 mM in TE buffer
- Andy Fluor 555-X-dUTP, 1 mM in TE buffer
- Andy Fluor 555-X-UTP, 1 mM in TE buffer
- Andy Fluor 568-X-dUTP, 1 mM in TE buffer
- Andy Fluor 568-X-UTP, 1 mM in TE buffer
- Andy Fluor 594-X-dUTP, 1 mM in TE buffer
- Andy Fluor 594-X-UTP, 1 mM in TE buffer
- Andy Fluor 647-X-dUTP, 1 mM in TE buffer
- Andy Fluor 647-X-UTP, 1 mM in TE buffer
- Cy3-X-dUTP, 1 mM in TE buffer
- Cy3-X-UTP, 1 mM in TE buffer
- Cy5-X-dUTP, 1 mM in TE buffer
- Cy5-X-UTP, 1 mM in TE buffer