Andy Fluor™ 488 Goat Anti-Mouse IgG (H+L) Antibody is prepared by labeling high quality goat anti-mouse IgG (H+L) with Andy Fluor™ 488. Andy Fluor™ 488 is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. Andy Fluor™ 488 can be used as a replacement for Alexa Fluor® 488.
Our EasyProbes™ secondary antibody reagent is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops to stain your cells.
Suggestions for use
• In most cases 2 drops/ml and an incubation of 30 minutes will give bright staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• Andy Fluor™ 488 dye is excited with a maximum at 495 nm, with an emission maximum at 520 nm. It is detected through standard GFP and FITC filters.
| Specifications | Form Information | ||||
| Host/Isotype: | Goat | Physical State: | Liquid | ||
| Class: | Polyclonal | Shipping Condition: | Ice pack | ||
| Type: | Secondary Antibody | Purification: | Immunoaffinity chromatography | ||
| Tested Species Reactivity: | Mouse (Ms) | Buffer: | PBS, pH 7.4, with 0.1% BSA | ||
| Target Class: | IgG (H+L) | Preservative: | 0.02% sodium azide | ||
| Antibody Form: | Whole Antibody | Storage Conditions: | 4ºC; store in dark | ||
| Label: | Andy Fluor™ 488 | ||||
| Excitation/Emission: | 495/520nm | ||||
| Unit Size: | 2 × 2.5 mL dropper bottles | ||||
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Immunofluorescent stain of α-tubulin and CCSP with Andy Fluor™ 488 Goat Anti-Mouse IgG (H+L) Antibody