Introduction
Features
Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The biotin azide is reactive with terminal alkyne via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.
Features
- Efficiency—the click reaction is complete in less than 1 hour;
- Specificity—the reaction between the label and detection tag is selective and specific;
- Stability—the reaction product contains an irreversible, covalent bond;
- Biologically inert—the components of the reaction do not undergo any side reactions.
Figure 1. Click chemistry labeling
Specifications
| Label: | Biotin |
|
| Ex/Em: | – | |
| Detection Method: | – | |
| Solubility: | DMSO, DMF | |
| Molecular Weight: | 400.50 | |
| Product Size: | 5 mg | |
| Storage Conditions: | -20 ℃, protect from light | |
| Shipping Condition: | Room Temperature | |
|
Applications Click chemistry labeling |
||
To order
Documents
| Datasheet (PDF): | C304 |
| MSDS (PDF): | C304 |
Reference
Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,
J Am Chem Soc (2008) 130:11576-11577
J Am Chem Soc (2008) 130:11576-11577
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,
J Am Chem Soc (2009) 131:4967-4975
J Am Chem Soc (2009) 131:4967-4975
Vila A, Tallman KA, Jacobs AT, Liebler DC, Porter NA, Marnett LJ,
Chem Res Toxicol (2008) 21:432-444
Chem Res Toxicol (2008) 21:432-444
Hsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH
Proc Natl Acad Sci U S A (2007) 104:2614-2619
Proc Natl Acad Sci U S A (2007) 104:2614-2619
Chang PV, Chen X, Smyrniotis C, Xenakis A, Hu T, Bertozzi CR, Wu P
Angew Chem Int Ed Engl (2009) 48:4030-4033
Angew Chem Int Ed Engl (2009) 48:4030-4033