BODIPY 576/589 NHS Ester (Succinimidyl Ester)

BODIPY 576/589 dye is bright, red fluorescent dye with similar excitation and emission to Rhodamine Red™ and Alexa Fluor™ 568 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. BODIPY 576/589 dye has a relatively long excited-state lifetime (typically 5 nanoseconds or longer), which is useful for fluorescence polarization-based assays and a large two-photon cross-section for multiphoton excitation. BODIPY dyes have unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.

The NHS ester (or succinimidyl ester) of BODIPY 576/589 is the most popular tool for conjugating the dye to a protein or antibody. NHS esters can be used to label the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting BODIPY 576/589 conjugates exhibit bright fluorescence, narrow emission bandwidths, and relatively long excited-state lifetimes, which can be useful for fluorescence polarization assays and two-photon excitation (TPE) microscopy.

Specifications

Label:	BODIPY 576/589
Ex/Em:	575/588 nm
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Chemical Reactivity:	Amine
Molecular Weight:	426.19
Quantity:	5 mg
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Fluorescent labeling