Our Cell-Quant™ MTT Cell Proliferation Assay Kit provides a standard cell proliferation analysis based on cellular metabolic activity. MTT is an widely established reagent that is used to determine cell number in a variety of applications. Live cells reduce MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader. This kit provides a complete package for performing a cell-counting assay that can detect from 2000 to 250,000 cells, depending on the cell type and conditions. The kit provides a sufficient amount of material for ~1000 assays.
|
Figure 1. Quantitation of Jurkat cells using the Cell-Quant™ MTT Cell Proliferation Assay Kit. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated for 4 hr with Cell-Quant™ MTT Cell Proliferation Assay Kit, absorbance was measured at 570 nm. |
Protocol (PDF): | A015 |
MSDS (PDF): | MSDS-A015 |
COA (PDF): | COA-A015 |
Reference:
Microb Pathog (1998) 25:203-214
Arch Pharm Res (1998) 21:353-356
Cell Transplant (1998) 7:443-451
- Cell-ID™ CFSE Cell Proliferation Kit
- Cell-ID™ Violet Cell Proliferation Kit
- iClick™ EdU Andy Fluor™ 488 Imaging Kit
- iClick™ EdU Andy Fluor™ 555 Imaging Kit
- iClick™ EdU Andy Fluor™ 594 Imaging Kit
- iClick™ EdU Andy Fluor™ 647 Imaging Kit
- iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit
- iClick™ EdU Andy Fluor™ 647 Flow Cytometry Assay Kit
- iClick™ EU Andy Fluor™ 488 Imaging Kit
- iClick™ EU Andy Fluor™ 594 Imaging Kit
- CCK-8
- Cell-Quant™ MTT Cell Proliferation Assay Kit
- Cell-Quant™ AlamarBlue Cell Viability Reagent