Introduction
Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite.
Specifications: | ||
Excitation/Emission: | 505/534 (oxidized form) | |
Shipping Condition: | Ice pack | |
Storage Conditions: | -20ºC, protect from light | |
Molecular Formula: | C20H18N2O3 | |
Molecular Weight: | 346.38 | |
CAS Number: | 109244-58-8 | |
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Documents
Protocol (PDF): | C261 |
MSDS (PDF): | MSDS-C261 |
COA (PDF): | COA-C261 |
Reference:
Reiter CD, Teng RJ, Beckman JS
J Biol Chem (2000) 275:32460-32466
J Biol Chem (2000) 275:32460-32466
Jourd'heuil D, Jourd'heuil FL, Kutchukian PS, Musah RA, Wink DA, Grisham MB
J Biol Chem (2001) 276:28799-28805
J Biol Chem (2001) 276:28799-28805
Miles AM, Bohle DS, Glassbrenner PA, Hansert B, Wink DA, Grisham MB
J Biol Chem (1996) 271:40-47
J Biol Chem (1996) 271:40-47
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