Introduction
Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca2+. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-3, AM is supplied as 1 mg solid (C213), special packing 20×50 μg (C214), and 1 mM solution in DMSO (C215).
Specifications
Label:
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Fluo-3
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Ex/Em:
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488/525
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Detection Method:
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Fluorescent
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Solubility:
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DMSO, DMF
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Molecular Formula:
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C51H50Cl2N2O23
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Molecular Weight:
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1129.85
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CAS Number:
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121714-22-5
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Storage Conditions:
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-20 ℃, protect from light
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Shipping Condition:
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Room Temperature
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Applications
Calcium indicator
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Documents
References:
Bikah G, Pogue-Caley RR, McHeyzer-Williams LJ, McHeyzer-Williams MG
Nat Immunol (2000) 1:402-412
duct usage:
Kriegler S, Chiu SY
J Neurosci (1993) 13:4229-4245oduct usage:
Lustig HS, von Brauchitsch KL, Chan J, Greenberg DA
J Neurochem (1992) 59:2193-2200