JC-1 Dye

JC-1 Dye (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ~590 nm. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green.

Specifications:
Excitation/Emission:	514/529 nm; 585/590 nm
Shipping Condition:	Ambient
Storage Conditions:	2-8ºC, protect from light
Molecular Formula:	C25H27Cl4IN4
Molecular Weight:	652.23
CAS Number:	47729-63-5