Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Andy Fluor™ 350. Andy Fluor™ 350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, Andy Fluor™ 350 is more water-soluble, more photostable and more fluorescent on proteins. Andy Fluor™ 350 can be used as a replacement for Alexa Fluor® 350.
Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Specifications | Form Information | |||||
Host/Isotype: | Goat | Physical State: | Lyophilized power | |||
Class: | Polyclonal | Shipping Condition: | Ambient | |||
Type: | Secondary Antibody | Purification: | Immunoaffinity chromatography | |||
Tested Species Reactivity: | Rabbit (Rb) | Buffer: | PBS, pH 7.4, with 0.1% BSA | |||
Target Class: | IgG (H+L) | Preservative: | 0.02% sodium azide | |||
Antibody Form: | Whole Antibody | Storage Conditions: | 4ºC; store in dark | |||
Label: | Andy Fluor™ 350 | |||||
Excitation/Emission: | 345/440 nm |
|
Immunofluorescent stain of α-tubulin with Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody |
|||
Unit Size: | 100 µg, 500 µg | |||||
Applications | Dilution | |||||
Western Blot (WB) | 1:5,000 – 1:20,000 | |||||
Immunofluorescence (IF) | 1:100 – 1:2,000 | |||||
Immunocytochemistry (ICC) | 1:100 – 1:2,000 | |||||
Immunohistochemistry (IHC) | 1:100 – 1:2,000 | |||||
Flow Cytometry (FACS) | 1:100 – 1:1,000 | |||||
- Andy Fluor 350 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor™ 405M Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor™ 405M Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 430 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 430 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 488 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 488 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 555 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 555 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 568 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 568 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 594 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 594 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 647 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 647 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor 680 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor 680 Goat Anti-Rabbit IgG (H+L) Antibody
- Andy Fluor™ 750 Goat Anti-Mouse IgG (H+L) Antibody
- Andy Fluor™ 750 Goat Anti-Rabbit IgG (H+L) Antibody