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Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody

Introduction

Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Andy Fluor™ 680. Andy Fluor™ 680 is superior in fluorescent brightness and photostability. Andy Fluor™ 680 can be used as a replacement for Alexa Fluor® 680 or Cy® 5.5 dye.

Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Specifications   Form Information
Host/Isotype: Goat   Physical State: Lyophilized power
Class: Polyclonal   Shipping Condition: Ambient
Type: Secondary Antibody   Purification: Immunoaffinity chromatography
Tested Species Reactivity: Rabbit (Rb)   Buffer: PBS, pH 7.4, with 0.1% BSA
Target Class: IgG (H+L)   Preservative: 0.02% sodium azide
Antibody Form: Whole Antibody   Storage Conditions: 4ºC; store in dark
Label: Andy Fluor 680    
Excitation/Emission: 680/700 nm

 

 

Immunofluorescent analysis of α-tubulin with Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody

 
Unit Size: 100 µg, 500 µg
   
Applications Dilution
Western Blot (WB) 1:5,000 – 1:20,000
Immunofluorescence (IF) 1:100 – 1:2,000
Immunocytochemistry (ICC) 1:100 – 1:2,000
Immunohistochemistry (IHC) 1:100 – 1:2,000
Flow Cytometry (FACS) 1:100 – 1:1,000
   

To Order  

Buy Cat.No. Product name Ex/Em (nm) Size Price

L129A
L129B
Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600

 

Documents

Protocol (PDF): L129A L129B
MSDS (PDF): L129A L129B
COA (PDF): L129A L129B