RedView™ is a sensitive, and stable fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for detection of dsDNA, ssDNA or RNA in agarose and polyacrylamide gels. This single stain gives more sensitive detection of dsDNA, ssDNA and RNA than EtBr. Gels can be post-stained or alternatively the stain can be added to agarose gels during gel casting. RedView™ has similar excitation and emission spectra with EtBr, and is compatible with EtBr imaging system.
RedView™ DNA Gel Stain, 10,000X is a concentrated RedView solution that can be diluted 10,000 times for use in precast gel staining or 5,000 times for use in post gel staining according to the procedures described below. One vial of 10,000X solution can be used to prepare at least 100 precast minigels or post-stain at least 100 minigels.
Gel staining with RedView™ is compatible with downstream applications such as gel extraction and cloning. RedView is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
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