Home » Cell Apoptosis Assay » TUNEL Chromogenic Apoptosis Detection Kit


TUNEL Chromogenic Apoptosis Detection Kit


Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavi­din) for fluorescence or colorimetric detection. Alternatively, fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry. The TUNEL Chromogenic Apoptosis Detection Kit contains dUTP conjugated to biotin and HRP-Streptavidin conjugate for high sensitive TUNEL staining.


  • Broad application: Suitable for FFPE tissue, frozen tissue and cells.
  • High sensitivity:Can detect a low population of apoptotic cells.


Platform: Light Microscopy
Detection Method: Colormetric
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Dry ice


Figure 1. Detection of apoptotic cells in mouse tongue tissue using  TUNEL Chromogenic Apoptosis Detection Kit.

To order

Buy Cat.No. Product Name Ex/Em Unit Price
A049-20T TUNEL Chromogenic Apoptosis Detection Kit 20 assays 1600
A049-50T TUNEL Chromogenic Apoptosis Detection Kit 50 assay 3500



Protocol (PDF): A049
COA (PDF):  COA-A049


Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.
Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H,
Genes Cells (2010) 15:315-325
Vasopressin inhibits apoptosis in renal collecting duct cells.
Miller RL, Sandoval PC, Pisitkun T, Knepper MA, Hoffert JD,
Am J Physiol Renal Physiol (2013) 304:F177-F188