5-FAM-X-dUTP, 1 mM solution

Introduction

5-FAM-X-dUTP (cat. no. C406A) is supplied as a 1 mM solution in TE buffer. 5-FAM-X-dUTP is also available in lyophilized form (cat. no. C406B).

5-FAM-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency.

Specifications:

Excitation/Emission (nm): 495/518 nm
Concentration: 1 mM solution in TE buffer
Storage: Store at -20°C
Molecular Formula: C₃₉H₃₈N₄Na₃O₂₁P₃
Molecular Weight: 1060.62

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.

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Documents

Protocol (PDF):	C406A
MSDS (PDF):	MSDS-C406A

Frequently asked questions (FAQs)

Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?
You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.

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Buy	Catalog #	Product Name	Ex/Em (nm)	Unit Size	List Price
	C406A	5-FAM-X-dUTP, 1 mM in TE buffer	495/518	25 µL	$180