5-FAM-X-dUTP (cat. no. C406B) is supplied in lyophilized form. 5-FAM-X-dUTP is also available as a 1 mM solution in TE buffer. (cat. no. C406A).
5-FAM-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency.
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Specifications:
Excitation/Emission (nm): 495/518 nm
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Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
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| Protocol (PDF): | C406B |
| MSDS (PDF): | MSDS-C406B |
Frequently asked questions (FAQs)
Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?
You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.
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