Protein Detection and Quantitation

Protein gel stain and Western Blot are important tool in proteomics (Figure 1). Applied BioProbes offers two distinct protein gel stains: 1) Coomassie® Blue Fast Stain, a ready-to-use product that does not require methanol or acetic acid fixatives or destains; 2) eLuminol™ Protein Gel Stain, a highly-sensitive fluorescent stain designed to detect proteins in SDS polyacrylamide gels. In addition, Applied BioProbes also offers a phosphoprotein selective stain: Phos-Tag™ Phosphoprotein Gel Stain. Applied BioProbes’ ECL Western Blotting Detection Kit provides highly sensitive detection on immunoblots. Besides, Applied BioProbes also offers two protein assay kits: 1) Bradford Protein Assay Kit; and 2) BCA Protein Assay Kit.

Figure 1. Protein gel analysis workflow

Protein Gel Stain Selection Guide

Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.

Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).