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核酸标记探针

产品介绍

ABP Biosciences 最新推出一系列荧光素和生物素标记dUTP, UTP和dCTP探针。这些探针可广泛应用于FISH, TUNEL, qPCR等应用。我们的探针采用荧光亮度强,光稳定性好的Andy Fluor™染料标记,并且在染料和dUTP及UTP之间设计一条特殊的X-linker以确保在酶的作用下更有效的插入DNA或RNA。

产品特点

◆荧光信号强,稳定性好,背景低;
◆多种颜色可供选择,适合多重染色;
◆适用性广:适用于各种酶的标记。​

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

TUNEL-biotin-11-UTP mouse tongue

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

订购

核酸标记探针

购买 货号 产品名称 激发/发射波长(nm) 规格 价格(¥)
C401 5-Aminoallyl-dUTP, 10 mM in TE buffer 100 µL 1200
C402 5-Aminoallyl-UTP, 10 mM in TE buffer 100 µL 1100
C403 Biotin-11-dUTP, 1 mM in TE buffer 50 µL 1800
C404 Biotin-11-UTP, 1 mM in TE buffer 50 µL 1800
C430 Biotin-14-dCTP, 0.4 mM 50 nmol 3500
C405 5-FAM-dUTP, 1 mM in TE buffer 495/518 25 µL 1400
C406 5-FAM-X-dUTP, 1 mM in TE buffer 495/518 25 µL 1800
C407 5-FAM-UTP, 1 mM in TE buffer 495/518 25 µL 1400
C408 5-FAM-X-UTP, 1 mM in TE buffer 495/518 25 µL 1800
C409 Andy Fluor 488-X-dUTP, 1 mM in TE buffer 500/525 25 µL 2800
C410 Andy Fluor 488-X-UTP, 1 mM in TE buffer 500/525 25 µL 2800
C411 Andy Fluor 555-X-dUTP, 1 mM in TE buffer 553/565 25 µL 2800
C412 Andy Fluor 555-X-UTP, 1 mM in TE buffer 553/565 25 µL 2800
C413 Andy Fluor 568-X-dUTP, 1 mM in TE buffer 578/602 25 µL 2800
C414 Andy Fluor 568-X-UTP, 1 mM in TE buffer 578/602 25 µL 2800
C415 Andy Fluor 594-X-dUTP, 1 mM in TE buffer 590/615 25 µL 2800
C416 Andy Fluor 594-X-UTP, 1 mM in TE buffer 590/615 25 µL 2800
C417 Andy Fluor 647-X-dUTP, 1 mM in TE buffer 650/665 25 µL 2800
C418 Andy Fluor 647-X-UTP, 1 mM in TE buffer 650/665 25 µL 2800
C419 Cy3-X-dUTP, 1 mM in TE buffer 553/565 25 µL 2400
C420 Cy3-X-UTP, 1 mM in TE buffer 553/565 25 µL 2400
C421 Cy5-X-dUTP, 1 mM in TE buffer 650/665 25 µL 2400
C422 Cy5-X-UTP, 1 mM in TE buffer 650/665 25 µL 2400
C423 Biotin-14-dATP 1mM in TE buffer   50nmol 3500
C424 Biotin-14-dGTP 1mM in TE buffer   25nmol 4800