Biotin-14-dCTP是一款dCTP类似物，在嘧啶基的N 4 –位通过一个14-原子连接头连接生物素。这种核苷酸能够在dATP，dGTP，和dTTP存在的条件下通过缺口平移有效地掺入DNA；或者用末端脱氧核苷酸转移酶 或者随机引物合成标记DNA。所提供的材料数量足够标记多达50 µg的 DNA。
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Specifications:
Concentration: 1 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. |
参考文献
Comparison of detection and signal amplification methods for DNA microarrays.Comparison of detection and signal amplification methods for DNA microarrays.
Vora GJ, Meador CE, Anderson GP, Taitt CR,
Mol Cell Probes (2008) 22:294-300
Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase.
Flickinger JL, Gebeyehu G, Buchman G, Haces A, Rashtchian A,
Nucleic Acids Res (1992) 20:2382-2382
Novel biotinylated nucleotide–analogs for labeling and colorimetric detection of DNA.Novel biotinylated nucleotide–analogs for labeling and colorimetric detection of DNA.
Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L
Nucleic Acids Res (1987) 15:4513-4534
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