EasyProbes™ DAPI是一种广泛用于固定细胞的细胞核染料,它可与双链DNA结合而发出蓝色荧光。DAPI常用于细胞核及染色体复染,可在染色体上显现一条明显的带型。EasyProbes™ DAPI Fixed Cell Stain是一种即用型的用于固定细胞的细胞核染料,染料储存在便捷的滴瓶中。使用时,只需按每毫升培养基滴加2滴染料,即可对细胞进行标记。
产品特点
- 无需计算、稀释、混匀等步骤
- 便捷滴瓶包装,每毫升培养基只需2滴染料
- 与DNA结合时,受紫外光激发,可在460 nm处发出蓝色荧光
使用建议
使用时,EasyProbes™ DAPI Fixed Cell Stain应直接滴入细胞培养基或缓冲液中。
- 大多数情况下,2滴/mL的比例、15-30分钟的孵育时间即可获得清晰的核染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
- EasyProbes™ DAPI Fixed Cell Stain与DNA结合时,最大激发波长为358 nm,其最大发射波长为460 nm。可通过蓝色滤光通道检测,如DAPI滤光通道。
Specifications: |
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Excitation/Emission: | 358/460 nm | |
Shipping Condition: | Ambient | |
Storage Conditions: | 4ºC, protect from light | |
Unit Size: | 10 mL |
Protocol (PDF): | FP026 |
MSDS (PDF): | FP026 |
COA (PDF): | FP026 |
Reference:
Chan B, VanderLaan PA, Sukhatme VP,
Biochem Biophys Res Commun (2013) 439:247-251
A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
Medin CL, Valois S, Patkar CG, Rothman AL,
J Virol Methods (2015) 211:55-62
Zhao N, Zhang AS, Worthen C, Knutson MD, Enns CA,
Proc Natl Acad Sci U S A (2014) -111(25):9175-80.
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