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EasyProbe™ DAPI Fixed Cell Stain

Introduction

EasyProbes™ DAPI是一种广泛用于固定细胞的细胞核染料,它可与双链DNA结合而发出蓝色荧光。DAPI常用于细胞核及染色体复染,可在染色体上显现一条明显的带型。EasyProbes™ DAPI Fixed Cell Stain是一种即用型的用于固定细胞的细胞核染料,染料储存在便捷的滴瓶中。使用时,只需按每毫升培养基滴加2滴染料,即可对细胞进行标记。

产品特点

  • 无需计算、稀释、混匀等步骤
  • 便捷滴瓶包装,每毫升培养基只需2滴染料
  • 与DNA结合时,受紫外光激发,可在460 nm处发出蓝色荧光

使用建议

使用时,EasyProbes™ DAPI Fixed Cell Stain应直接滴入细胞培养基或缓冲液中。

  • 大多数情况下,2滴/mL的比例、15-30分钟的孵育时间即可获得清晰的核染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
  • EasyProbes™ DAPI Fixed Cell Stain与DNA结合时,最大激发波长为358 nm,其最大发射波长为460 nm。可通过蓝色滤光通道检测,如DAPI滤光通道。
Specifications:  
Excitation/Emission: 358/460 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 10 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP026 EasyProbe™ DAPI Fixed Cell Stain 358/460 10 mL 600

Documents

Protocol (PDF): FP026
MSDS (PDF):  FP026
COA (PDF):  FP026

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J Virol Methods (2015) 211:55-62


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Zhao N, Zhang AS, Worthen C, Knutson MD, Enns CA, 
Proc Natl Acad Sci U S A (2014) -111(25):9175-80.