EasyProbe™ Green 488 Dead Cell Stain是一种细胞不可渗透性染料,它可与死细胞内的细胞核DNA结合而发出明亮的绿色荧光。EasyProbe™ Green 488 Dead Cell Stain是一种即用型的用于死细胞的细胞核染料,染料储存在便捷的滴瓶中。使用时,只需按每毫升培养基滴加2滴染料,即可对细胞进行标记。
产品特点
- 无需计算、稀释、混匀等步骤
- 便捷滴瓶包装,每毫升培养基只需2滴染料
- 无需洗涤步骤,即可快速对细胞或组织染色
使用建议
使用时,EasyProbes™ Green 488 Dead Cell Stain应直接滴入细胞培养基或缓冲液中。
- 大多数情况下,2滴/mL的比例、15-30分钟的孵育时间即可获得清晰的核染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
- EasyProbes™ Green 488 Dead Cell Stain与DNA结合时,最大激发波长为500 nm,最大发射波长为530 nm。其荧光可通过标准GFP或FITC滤光通道检测。
Specifications: | ||
Excitation/Emission: | 500/530 nm | |
Shipping Condition: | Ambient | |
Storage Conditions: | 4ºC, protect from light | |
Unit Size: | 10 mL |
Protocol (PDF): | FP030 |
MSDS (PDF): | FP030 |
COA (PDF): | FP030 |
Reference:
Davies BR, Greenwood H, Dudley P, Crafter C, Yu DH, Zhang J, Li J, Gao B, Ji Q, Maynard J, Ricketts SA, Cross D, Cosulich S, Chresta CC, Page K, Yates J, Lane C, Watson R, Luke R, Ogilvie D, Pass M,
Mol Cancer Ther (2012) 11:873-887
The G1 cyclin Cln3p controls vacuolar biogenesis in Saccharomyces cerevisiae.
Han BK, Aramayo R, Polymenis M
Genetics (2003) 165:467-476
Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR.
Hodson JA, Bailis JM, Forsburg SL
Nucleic Acids Res (2003) 31:e134-e134
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