EasyProbe™ ActinGreen 488 Stain是一种对F-肌动蛋白具有高亲和性和高选择性的染料,它由Andy Flour™ 488荧光染料与鬼笔环肽偶联而成。EasyProbes™ ActinGreen 488 Stain提供了一种即用型的细胞骨架染色方案,染料储存在便捷的滴瓶中。使用时,只需在细胞玻片上滴2滴染液,即可使细胞染色。
产品特点
- 对F-actin具有高亲和性,优于抗体染色
- 无需计算、稀释、混匀等步骤
- 荧光信号强
使用建议
使用时,EasyProbes™ ActinGreen 488 Stain应直接滴在细胞玻片上,覆盖所有细胞。
- 大多数情况下,2滴染料、15-30分钟的孵育时间即可获得清晰的染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
- EasyProbes™ ActinGreen 488 Stain的最大激发波长为500 nm,最大发射波长为525 nm。其荧光可通过标准GFP或FITC滤光片检测。
Specifications: | ||
Excitation/Emission: | 500/525 nm | |
Shipping Condition: | Ambient | |
Storage Conditions: | 4ºC, protect from light | |
Unit Size: | 5 mL |
Protocol (PDF): | FP031 |
MSDS (PDF): | FP031 |
COA (PDF): | FP031 |
Reference:
Joshi B, Bastiani M, Strugnell SS, Boscher C, Parton RG, Nabi IR,
J Cell Biol (2012) 199:425-435
Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties.
Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE,
PLoS One (2008) 3:e3077-e3077
Schimizzi GV, Currie JD, Rogers SL,
PLoS One (2010) 5:e11381-e11381
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