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EasyProbe™ ActinGreen 488 Stain

Introduction

EasyProbe™ ActinGreen 488 Stain是一种对F-肌动蛋白具有高亲和性和高选择性的染料,它由Andy Flour™ 488荧光染料与鬼笔环肽偶联而成。EasyProbes™ ActinGreen 488 Stain提供了一种即用型的细胞骨架染色方案,染料储存在便捷的滴瓶中。使用时,只需在细胞玻片上滴2滴染液,即可使细胞染色。

产品特点

  • 对F-actin具有高亲和性,优于抗体染色
  • 无需计算、稀释、混匀等步骤
  • 荧光信号强


使用建议

使用时,EasyProbes™ ActinGreen 488 Stain应直接滴在细胞玻片上,覆盖所有细胞。

  • 大多数情况下,2滴染料、15-30分钟的孵育时间即可获得清晰的染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
  • EasyProbes™ ActinGreen 488 Stain的最大激发波长为500 nm,最大发射波长为525 nm。其荧光可通过标准GFP或FITC滤光片检测。
Specifications:  
Excitation/Emission: 500/525 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 5 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP031 EasyProbe™ ActinGreen 488 Stain 500/525 5 mL 1500

Documents

Protocol (PDF): FP031
MSDS (PDF):  FP031
COA (PDF):  FP031

Reference:

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J Cell Biol (2012) 199:425-435


Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties.

Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE, 
PLoS One (2008) 3:e3077-e3077


Expression levels of a kinesin-13 microtubule depolymerase modulates the effectiveness of anti-microtubule agents.

Schimizzi GV, Currie JD, Rogers SL, 
PLoS One (2010) 5:e11381-e11381