EasyProbe™ ActinRed 555 Stain是一种对F-肌动蛋白具有高亲和性和高选择性的染料,它由橙红色荧光染料四甲基罗丹明(TMR)与鬼笔环肽偶联而成。EasyProbes™ ActinRed 555 Stain提供了一种即用型的细胞骨架染色方案,染料储存在便捷的滴瓶中。使用时,只需在细胞玻片上滴2滴染液,即可使细胞染色。
产品特点
- 对F-actin具有高亲和性,优于抗体染色
- 无需计算、稀释、混匀等步骤
- 荧光信号强
使用建议
使用时,EasyProbe™ ActinRed 555 Stain应直接滴在细胞玻片上,覆盖所有细胞。
- 大多数情况下,2滴染料、15-30分钟的孵育时间即可获得清晰的染色效果。然而,对于某些特殊的细胞类型、实验条件及研究应用等,需要优化染色方案。
- EasyProbes™ ActinRed 555的最大激发波长为540 nm,最大发射波长为565 nm。其荧光可通过标准TRITC滤光通道检测。
Specifications: |
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Excitation/Emission: | 555/575 nm | |
Shipping Condition: | Ambient | |
Storage Conditions: | 4ºC, protect from light | |
Unit Size: | 5 mL |
Protocol (PDF): | FP032 |
MSDS (PDF): | FP032 |
COA (PDF): | FP032 |
Reference:
She X, Chen L, Velleman L, Li C, Zhu H, He C, Wang T, Shigdar S, Duan W, Kong L,
J Colloid Interface Sci (2015) 445:151-160
In situ measurement of magnetization relaxation of internalized nanoparticles in live cells.
Soukup D, Moise S, Céspedes E, Dobson J, Telling ND,
ACS Nano (2015) 9:231-240
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