5-Aminoallyl-dUTP (AA-dUTP), lyophilized powder

5-Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) (cat. no. C401B) is supplied in lyophilized form. 5-Aminoallyl-dUTP is also available as a 10 mM solution in TE buffer. (cat. no. C401A).

5-Aminoallyl-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA polymerase, phi29 DNA Polymerase, Klenow Fragment, Klenow Fragment, exo- and DNA Polymerase I. The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled dUTP.

Specifications: Storage: Store at -20°C Molecular Formula: C12H17N3Na3O14P3 Molecular Weight: 589.17

Applications

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension.

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