5-Aminoallyl-UTP (AA-UTP), lyophilized powder

5-Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) (cat. no. C402B) is supplied in inlyophilized form. 5-Aminoallyl-UTP is also available as a 10 mM solution in TE buffer. (cat. no. C402A).

5-Aminoallyl-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases. The resulting amine-containing RNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten. This two-step method for labeling nucleic acids is considerably more economical than the one-step method using a prelabeled UTP.

Specifications: Storage: Store at -20°C Molecular Formula: C12H17N3Na3O15P3 Molecular Weight: 605.17

Applications

Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random-primed labeling, or primer extension

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