Introduction
|
Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell. |
Specifications
| Label: | 5-(and 6)-Carboxy-2',7'-dichlorofluorescein |
|
| Ex/Em: | 495/529 nm | |
| Detection Method: | Fluorescent | |
| Solubility: | DMSO, DMF | |
| Molecular Formula: | C25H16Cl2O9 | |
| Molecular Weight: | 531.30 | |
| CAS Number: | – | |
| Storage Conditions: | -20 ℃, protect from light | |
| Shipping Condition: | Room Temperature | |
|
Applications Probe for ROS |
||
To order
Documents
| Datasheet (PDF): | C264 |
| MSDS (PDF): | MSDS-C264 |
References:
Toh PP, Luo S, Menzies FM, Raskó T, Wanker EE, Rubinsztein DC,
Hum Mol Genet (2013) 22:5237-5248
Hum Mol Genet (2013) 22:5237-5248
Visualization and quantitation of cyclooxygenase-1 and -2 activity by digital fluorescence microscopy.
Ornberg RL, Koki AT
Eicosanoids and Other Bioactive Lipids in Cancer Inflammation, and Radiation Injury, 4, Honn KV, Mar 1999; (na):na pp. 131-137
Eicosanoids and Other Bioactive Lipids in Cancer Inflammation, and Radiation Injury, 4, Honn KV, Mar 1999; (na):na pp. 131-137
Uchida Y, Ohba K, Yoshioka T, Irie K, Muraki T, Maru Y
J Biol Chem (2004) 279:4075-4083
J Biol Chem (2004) 279:4075-4083