ROS Assay Kit

Reactive oxygen species assay kit uses a unique fluorescent probe H₂DCFDA to detect reactive oxygen species. H₂DCFDA is chemically reduced and acetylated form of 2′,7′-dichloro- fluorescein (DCF) and is nonfluorescent and cell-permeant.   

Once H₂DCFDA enters into cells, the acetate groups are removed by intracellular esterases to form H₂DCF. H₂DCF is well retained inside cells. Oxidation of H₂DCF by reactive oxygen species yields fluorescent DCF. Reactive oxygen species can be detected by monitoring the increase in fluorescence with a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein (FITC). Because the dyes are susceptible to photo-oxidation, low light conditions should be used for fluorescence microscopy applications whenever possible.