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Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells

Introduction

The Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells provides two-color fluorescence staining on both live (green) and dead (red) bacteria using two probes, SYTO 9 dye and Propidium iodide. SYTO 9 dye is a green-fluorescent nucleic acid dye that stains both live and dead bacteria with intact and damaged cell membranes. Propidium iodide is a red-fluorescent nucleic acid dye that stains only dead bacteria with damaged cell membranes. With an appropriate mixture of SYTO 9 and Propidium iodide, bacteria with intact cell membranes is stained fluorescent green, whereas bacteria with damaged cell membranes is stained fluorescent red. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types.

Features:

  • Simplicity: Reagents are simultaneously added, no wash steps are required
  • Specificity and reliability: Distinct color for live and dead cell
  • Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader

Specifications
Platform: Fluorescence Microscopy, Flow Cytometry
 
Detection Method: Fluorescent
Ex/Em: SYTO 9 dye: 480/500; PI: 535/617 nm
Product Size: 1000 assays
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room temperature

Applications

Cell viability assay
 


Figure 1. Live and dead E.coli cells stained with Cell-Check™ Viability/Cytotoxicity Kit (A018). Live bacteria exhibit green fluorescence, whereas dead bacteria exhibit red fluorescence.

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
A018 Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells 1000 assays 1800
 

Documents

 

Protocol (PDF): A018
MSDS (PDF):  MSDS-A018
COA (PDF): COA-A018


Cited Reference:

Antibacterial effect of bacteriocin XJS01 and its application as antibiofilm agents to treat multidrug-resistant Staphylococcus aureus infection
Yi-Zhou Xiang, Gang Wu, Lin-Yu Yang, Xiao-Jie Yang, Yan-Mei Zhang, Lian-Bing Lin, Xian-Yu Deng, Qi-Lin Zhang
International Journal of Biological Macromolecules, 2022, 196, 13-22, https://doi.org/10.1016/j.ijbiomac.2021.11.136


A novel bacteriocin against Staphylococcus aureus from Lactobacillus paracasei isolated from Yunnan traditional fermented yogurt: Purification, antibacterial characterization, and antibiofilm activity
Yu-Hang Jiang, Wei-Gang Xin, Lin-Yu Yang, Jian-Ping Ying, Zi-Shun Zhao, Lian-Bing Lin, Xiu-Zhang Li, Qi-Lin Zhang
Journal of Dairy Science, 2022, 105(3):2094-2107. https://doi.org/10.3168/jds.2021-21126


Antibacterial activity and action target of phenyllactic acid against Staphylococcus aureus and its application in skim milk and cheese
Yu-Hang Jiang, Jian-Ping Ying, Wei-Gang Xin, Lin-Yu Yang, Xiu-Zhang Li, Qi-Lin Zhang
J. Dairy Sci. 2022, 105:9463–9475. https://doi.org/10.3168/jds.2022-22262


Purification, characterization, and antibacterial and antibiofilm activity of a novel bacteriocin against Salmonella Enteritidis.
Xiang, Y.-Z., Zhang, Y.-M., Liu, Y.-Y., Zhang, M., Lin, L.-B., & Zhang, Q.-L.
Food Control, 2021, 127, 108110. doi:10.1016/j.foodcont.2021.10


A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity.
Jiang Y-H, Xin W-G, Zhang Q-L, Lin L-B and Deng X-Y
Front. Microbiol. 2022, 12:779315. doi: 10.3389/fmicb.2021.779315


Effect of surface sealant on surface roughness and bacterial adhesion of bulk-fill composites
Gunce Ozan, Meltem Mert Eren, Cansu Vatansever and Ugur Erdemir
Polymers and Polymer Composites, 2021, Vol. 29(9S) S475–S484. DOI: 10.1177/09673911211005586


Antibacterial activity and mechanism of action of bacteriocin LFX01 against Staphylococcus aureus and Escherichia coli and its application on pork model
Wei-Gang Xin, Gang Wu, Jian-Ping Ying, Yi-Zhou Xiang, Yu-Hang Jiang, Xian-Yu Deng, Lian-Bing Lin, Qi-Lin Zhang
Meat Science, 2023, 196, 109045, https://doi.org/10.1016/j.meatsci.2022.109045


Streptococcus Mutans adhesion to dental restorative materials after polishing with varioussystems:AConfocal Microscopy study
M.MertErena,G.Ozanb,U.Erdemirb,C.Vatansever
Acta Microscópica, 2021, 30, 1, 53-64


A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity
Hong-Wei Li, Yi-Zhou Xiang, Man Zhang, Yu-Hang Jiang, Yao Zhang, Ying-Yang Liu, Lian-Bing Lin, Qi-Lin Zhang
LWT, 2021, 140, 110826, https://doi.org/10.1016/j.lwt.2020.110826