Andy Fluor™ 555 Annexin V is Annexin V conjugated with our orange fluorescent dye Andy Fluor™ 555. Annexin V is a calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding.
Andy Fluor™ 555 is a superior alternative to the traditional orange fluorescent dye TAMRA owing to its exceptional brightness and photostability. Andy Fluor™ 555 has brightness and photostability comparable to Alexa Fluor® 555.
Our Andy Fluor™ 555 Annexin V is provided as lyophilized power, which is enough for 100 assays.
Reconstitution: We recommend a quick spin followed by reconstitution in 500 μl distilled water, and store at 4°C.
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| Protocol (PDF): | A035 |
| MSDS (PDF): | MSDS-A035 |
| COA (PDF): | COA-A035 |
Reference
Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
Logue SE, Elgendy M, Martin SJ,
Nat Protoc (2009) 4:1383-1395
Flow cytometry-based apoptosis detection.
Wlodkowic D, Skommer J, Darzynkiewicz Z,
Methods Mol Biol (2009) 559:19-32
Fluorescent biomembrane probe for ratiometric detection of apoptosis.
Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
J Am Chem Soc (2007) 129:2187-2193
Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
Pläsier B, Lloyd DR, Paul GC, Thomas CR, Al-Rubeai M
J Immunol Methods (1999) 229:81-95
- eGFP Annexin V and PI Apoptosis Kit
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- Annexin V, recombinant
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- Andy Fluor™ 647 Annexin V
- Andy Fluor™ 680 Annexin V