DNA含量的测定可以用来研究细胞在各阶段周期的分布以及DNA倍体的分析。在给定群体中,细胞主要分布在细胞周期的三个阶段:G0 / G1期,S期和G2 / M期。在G2 / M期,细胞含双份基因组DNA,是G0 / G1期细胞的2倍,而正在进行DNA复制的S期细胞的DNA含量介于二者之间。当选用DNA选择性染料来测量DNA含量时,其荧光强度与DNA含量成正比。细胞内的DNA被染色后,可以用流式细胞仪对细胞进行DNA含量测定,然后根据DNA含量的分布情况,可以进行细胞周期分析。
CellCycle™ Green染色液是一种操作简单方便的DNA选择性染料。只需将其添加到活细胞中(无需固定),孵育后直接在流式细胞仪上进行细胞周期分析。
Specifications: | ||
Excitation/Emission: | 500/530 nm | |
Shipping Condition: | Ambient | |
Storage Conditions: | 4ºC, protect from light | |
Unit Size: | 100 assays |
Protocol (PDF): | A054 |
MSDS (PDF): | A054 |
COA (PDF): | A054 |
Cited Reference:
PAQR3 Inhibits Non-small Cell Lung Cancer Growth by Regulating the NF-κB/p53/Bax Axis
Guo Q, Ke X-X, Fang S-X, Gao W-L, Song Y-X, Chen C, Lu H-L and Xu G
Front. Cell Dev. Biol. 2020, 8:581919. doi: 10.3389/fcell.2020.581919
[11]-chaetoglobosins with cytotoxic activities from Pseudeurotium bakeri
Fangfang Duan, Ying Gao, Xiaogang Peng, Xianggao Meng, Jinling Chang, Yutian Gan, Qianxi Ouyang, Hanli Ruan
Bioorganic Chemistry, 2022, 127, 106011, https://doi.org/10.1016/j.bioorg.2022.106011
Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer
Zhai, L., Chen, W., Cui, B., Yu, B., Wang, Y., & Liu, H.
Tissue and Cell, 2021, 73, 101611. doi:10.1016/j.tice.2021.101611
[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
SSRN: http://dx.doi.org/10.2139/ssrn.4102564