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CellCycle™ Ruby Stain

Introduction

DNA含量的测定可以用来研究细胞在各阶段周期的分布以及DNA倍体的分析。在给定群体中,细胞主要分布在细胞周期的三个阶段:G0 / G1期,S期和G2 / M期。在G2 / M期,细胞含双份基因组DNA,是G0 / G1期细胞的2倍,而正在进行DNA复制的S期细胞的DNA含量介于二者之间。当选用DNA选择性染料来测量DNA含量时,其荧光强度与DNA含量成正比。细胞内的DNA被染色后,可以用流式细胞仪对细胞进行DNA含量测定,然后根据DNA含量的分布情况,可以进行细胞周期分析。

CellCycle™ Ruby染色液是一种操作简单方便的DNA选择性染料。只需将其添加到活细胞中(无需固定),孵育后直接在流式细胞仪上进行细胞周期分析。

 

Specifications:  
Excitation/Emission: 647/680 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 100 assays

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
A055 CellCycle™ Ruby Stain 647/680 100 assays 1600

Documents

 

Protocol (PDF): A055
MSDS (PDF):  A055
COA (PDF):  A055


Cited Reference:

PAQR3 Inhibits Non-small Cell Lung Cancer Growth by Regulating the NF-κB/p53/Bax Axis
Guo Q, Ke X-X, Fang S-X, Gao W-L, Song Y-X, Chen C, Lu H-L and Xu G
Front. Cell Dev. Biol. 2020, 8:581919. doi: 10.3389/fcell.2020.581919


[11]-chaetoglobosins with cytotoxic activities from Pseudeurotium bakeri
Fangfang Duan, Ying Gao, Xiaogang Peng, Xianggao Meng, Jinling Chang, Yutian Gan, Qianxi Ouyang, Hanli Ruan
Bioorganic Chemistry, 2022, 127, 106011, https://doi.org/10.1016/j.bioorg.2022.106011


Overexpressed versican promoted cell multiplication, migration and invasion in gastric cancer
Zhai, L., Chen, W., Cui, B., Yu, B., Wang, Y., & Liu, H.
Tissue and Cell, 2021, 73, 101611. doi:10.1016/j.tice.2021.101611


[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
​SSRN: http://dx.doi.org/10.2139/ssrn.4102564