Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment.
In the TUNEL assay, TdT enzyme catalyzes the addition of modified dUTP to the 3’-OH ends of fragmented DNA. The modifications are fluorophores or haptens, including biotin or bromine which can be detected directly in the case of a fluorescently-modified nucleotide, or indirectly with streptavidin or antibodies, if biotin-dUTP or BrdUTP are used, respectively.
The new iClick™ TUNEL Andy Fluor™ 488 Imaging Assay utilize a dUTP modified with an alkyne, a small, bio-orthogonal functional group that enables the nucleotide to be more readily incorporated by TdT than other modified nucleotides such as biotin-dUTP and fluorescein-dUTP. Detection is based on a click reaction, a copper (I) catalyzed reaction between an azide and alkyne (Figure 1). The small size of the Andy Fluor™ azide (MW ∼1,000) compared to that of an antibody (MW ∼150,000) enables effortless penetration of complex samples with only mild fixation and permeabilization required. As a result, when compared to assays using other modified nucleotides, the iClick™ TUNEL Imaging Assay is fast (complete within 2 hours) and is able to detect a higher percentage of apoptotic cells under identical conditions.
Features
- Broad application: Suitable for FFPE tissue, frozen tissue and cells.
- High sensitivity: Can detect a higher percentage of apoptotic cells.
- Bright apoptotic signal: Uses Andy Fluor™ dyes, resulting in a stable, non-photobleaching fluorescent signal.
Figure 1. Detection of apoptosis with the iClick™ TUNEL Imaging Assay.
|
|
Protocol (PDF): | A060 | |
MSDS (PDF): |
|
|
Cited Reference:
DSTYK Promotes Metastasis and Chemoresistance via EMT in Colorectal Cancer
Zhang J, Miller Z, Musich PR, Thomas AE, Yao ZQ, Xie Q, Howe PH and Jiang Y
Front. Pharmacol., 2020, 11:1250. doi: 10.3389/fphar.2020.01250
A Novel Drug Combination of Mangiferin and Cinnamic Acid Alleviates Rheumatoid Arthritis by Inhibiting TLR4/NF?B/NLRP3 Activation-Induced Pyroptosis
Weijie Li, Kexin Wang, Yudong Liu, Hao Wu, Yan He, Congchong Li, Qian Wang, Xiaohui Su, Shikai Yan, Weiwei Su, Yanqiong Zhang, Na Lin
Front Immunol. 2022; 13: 912933. doi: 10.3389/fimmu.2022.912933
Disease-Modifying Anti-rheumatic Drug Prescription Baihu-Guizhi Decoction Attenuates Rheumatoid Arthritis via Suppressing Toll-Like Receptor 4-mediated NLRP3 Inflammasome Activation
Li W, Mao X, Wang X, Liu Y, Wang K, Li C, Li T, Zhang Y and Lin N
Front. Pharmacol. 2021, 12:743086. doi: 10.3389/fphar.2021.743086
MicroRNA miR-215-5p Regulates Doxorubicin-induced Cardiomyocyte Injury by Targeting ZEB2
Xiong, Xiaojv MD; He, Qin BSMed; Liu, Jinhua MM; Dai, Rui MM; Zhang, Hui MM; Cao, Zhe MM; Liao, Yuanxi MD; Liu, Bo MD; Zhou, Yi MM; Chen, Juan MD; Chen, Manhua MM; Liu, Jijun MM
Journal of Cardiovascular Pharmacology, 2021, 78(4):622-629. DOI: 10.1097/FJC.0000000000001110
Effects of amantadine and topiramate on neuronal damage in rats with experimental cerebral ischemia-reperfusion
Gündüz ZB, Aktas F, Vatansev H, Solmaz M, Erdoan E
Adv Clin Exp Med. 2021;30(10):1013–1023. doi:10.17219/acem/138327
DSTYK Enhances chemoresistance in triple-Negative breast cancer cells
Ogbu, S.C.; Rojas, S.; Weaver, J.; Musich, P.R.; Zhang, J.; Yao, Z.Q.; Jiang, Y.
Cells 2022, 11(1), 97; https://doi.org/10.3390/cells11010097
2-Methoxyestradiol Damages DNA in Glioblastoma Cells by Regulating nNOS and Heat Shock Proteins
Bastian, P.E.; Daca, A.; Poska, A.; Kuban-Jankowska, A.; Kalinowski, L.; Gorska-Ponikowska, M.
Antioxidants 2022, 11(10), 2013; https://doi.org/10.3390/antiox11102013
A ROCK inhibitor promotes keratinocyte survival and paracrine secretion, enhancing establishment of primary human melanocytes and melanocyte–keratinocyte co-cultures
Mi, J., Feng, Y., Wen, J., Su, Y., Xu, L., Zu, T., … Wu, X.
Pigment Cell & Melanoma Research, 2019, 33(1), 16–29. doi:10.1111/pcmr.12816
Effect of Trigonelline in a Model of Apoptosis in Rat Retina
SA Postacı, Ü Çeliker, N lhan, F Dalı, A Dal
Research Square, 2021. https://doi.org/10.21203/rs.3.rs-837275/v1
Disruption of C/EBP?-Clec7a axis exacerbates neuroinflammatory injury via NLRP3 inflammasome-mediated pyroptosis in experimental neuropathic pain
Wu, D., Zhang, Y., Zhao, C. et al.
J Transl Med 20, 583 (2022). https://doi.org/10.1186/s12967-022-03779-9
Evaluation of the Neuroprotective Effect of Pycnogenol in a Hypoxic-Ischemic Brain Injury Model in Newborn Rats
Ruya Çolak, Aslı Celik, Gulden Diniz, Senem Alkan Özdemir, Osman Yilmaz, Sebnem Calkavur
Am J Perinatol, 2021, DOI: 10.1055/s-0041-1730349
The therapeutic effect of Cilastatin on drug-induced nephrotoxicity: a new perspective
T. BECERIR, O. TOKGÜN, S. YUKSEL
European Review for Medical and Pharmacological Sciences, 2021, 25:5436-5447.
Dependence of visual and cognitive outcomes on animal holder configuration in a rodent model of blast overpressure exposure
Rachael S. Allen, Cara T. Motz, Anayesha Singh, Andrew Feola, Lauren Hutson, Amber Douglass, Sriganesh Ramachandra Rao, Lara A. Skelton, Lidia Cardelle, Katie L. Bales, Kyle Chesler, Kaavya Gudapati, C. Ross Ethier, Matthew M. Harper, Steven J. Fliesler, Machelle T. Pardue
Vision Research, 2021, 188, 162-173, https://doi.org/10.1016/j.visres.2021.07.008
Resolving the conflicts around Par2 opposing roles in regeneration by comparing immune-mediated and toxic-induced injuries.
Reches, G., Blondheim Shraga, N.R., Carrette, F. et al.
Inflamm Regener 42, 52 (2022). https://doi.org/10.1186/s41232-022-00238-2
The effect of platelet-rich plasma on chondrocyte healing in traumatic dislocation of the hip in a rat model
Toktamı Sava, Orhan Büyükbebeci, Erman Öümsöütlü, Ahmet Sarper Bozkurt, Ömer Eronat
J Surg Med. 2021;5(12):1159-1163. DOI: 10.28982/josam.1005052
The effect of hypothermia in a rat testicular torsion/detorsion model
Süleyman Cüneyt Karaku, Alev Süzen, Çidem Yenisey, Nazile Ertürk, Turul Epikmen, Emrah pek, dil Rana User, Burçin rem Abas
Journal of Pediatric Urology, 2021, 17, 3, 291.e1-291.e8, https://doi.org/10.1016/j.jpurol.2021.01.045
Therapeutic Effect of Teneligliptin in Drug-Induced Nephrotoxicity: An In-Vitro Study
Becerir T, Tokgün O, ci K, et al.
Cureus 2022, 14(4): e23871. doi:10.7759/cureus.23871
Zinc sulfide-based hybrid exosome-coated nanoplatform for targeted treatment of glioblastoma in an orthotopic mouse glioblastoma model
Wenxu Liu, Lili Wei, Meiying Li, Jingxin Mo
Materials Today Advances, 2023, 17, 100327, https://doi.org/10.1016/j.mtadv.2022.100327
Traf2 and NCK Interacting Kinase Is a Critical Regulator of Procollagen I Trafficking and Hepatic Fibrogenesis in Mice
Buchl, S.C., Hanquier, Z., Haak, A.J., Thomason, Y.M., Huebert, R.C., Shah, V.H. and Maiers, J.L.
Hepatol Commun, 2022, 6: 593-609. https://doi.org/10.1002/hep4.1835
Oxygen-Enriched Metal-Phenolic X-Ray Nanoprocessor for Cancer Radio-Radiodynamic Therapy in Combination with Checkpoint Blockade Immunotherapy
Sang, W., Xie, L., Wang, G., Li, J., Zhang, Z., Li, B., Guo, S., Deng, C.-X., Dai, Y.
Adv. Sci. 2021, 8, 2003338. https://doi.org/10.1002/advs.202003338
Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer
Zainab Shonibare, Mehri Monavarian, Kathleen O’Connell, Diego Altomare, Abigail Shelton, Shubham Mehta, Renata Jaskula-Sztul, Rebecca Phaeton, Mark D. Starr, Regina Whitaker, Andrew Berchuck, Andrew B. Nixon, Rebecca C. Arend, Nam Y. Lee, C. Ryan Miller, Nadine Hempel, Karthikeyan Mythreye
Cell Reports, 2022, 40, 4, 111066, https://doi.org/10.1016/j.celrep.2022.111066
ATP6AP2 knockdown in cardiomyocyte deteriorates heart function via compromising autophagic flux and NLRP3 inflammasome activation
Li, L., Cui, Yj., Liu, Y. et al.
Cell Death Discov. 8, 161 (2022). https://doi.org/10.1038/s41420-022-00967-w
A Dhdds K42E knock-in RP59 mouse model shows inner retina pathology and defective synaptic transmission
M Nguyen, D Chakraborty, SR Rao, A Onysk
Research Square, 2022, https://doi.org/10.21203/rs.3.rs-2054399/v1
Recombinant EGFL7 Mitigated Pressure Overload-Induced Cardiac Remodeling by Blocking PI3K/AKT/ Signaling in Macrophages
Li L, Zhao Y, Hu Y, Wang X, Jin Q, Zhao Y.
Front Pharmacol. 2022 May 26;13:858118. doi: 10.3389/fphar.2022.858118.
LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma
Lukas Leiendecker, Pauline S Jung, Izabela Krecioch, Tobias Neumann, Alexander Schleiffer, Karl Mechtler, Thomas Wiesner, Anna C Obenauf
EMBO Mol Med (2020) 12: e12525https://doi.org/10.15252/emmm.202012525
Zinc sulfide-based hybrid exosome-coated nanoplatform for targeted treatment of glioblastoma in an orthotopic mouse glioblastoma model
Wenxu Liu, Lili Wei, Meiying Li, Jingxin Mo
Materials Today Advances, 2023, 17, 100327, https://doi.org/10.1016/j.mtadv.2022.100327
Disruption of C/EBP β-Clec7a axis exacerbates neuroinflammatory injury via NLRP3 inflammasome-mediated pyroptosis in experimental neuropathic pain.
Wu, D., Zhang, Y., Zhao, C. et al.
J Transl Med 20, 583 (2022). https://doi.org/10.1186/s12967-022-03779-9
Related Products
- TUNEL Chromogenic Apoptosis Detection Kit
- TUNEL Andy Fluor™ 488 Apoptosis Detection Kit
- TUNEL Andy Fluor™ 594 Apoptosis Detection Kit
- TUNEL Andy Fluor™ 647 Apoptosis Detection Kit
- iClick™ TUNEL Andy Fluor™ 488 Imaging Assay
- iClick™ TUNEL Andy Fluor™ 555 Imaging Assay
- iClick™ TUNEL Andy Fluor™ 594 Imaging Assay
- iClick™ TUNEL Andy Fluor™ 647 Imaging Assay