iQuant™ microRNA Assay Kit 是一款定量小RNA, 包括单链或双链 microRNA 和 siRNA 的定量检测试剂盒。该试剂盒对小RNA的选择性高于rRNA或大mRNA。与其他传统的 RNA 定量相比，该检测方法具有稳定性好，线性范围宽及检测灵敏度高等优点。试剂盒包含浓缩的定量检测试剂、稀释缓冲液以及预稀释的microRNA标准品。您只需用试剂盒提供的缓冲液稀释定量检测试剂，加入待检测样品（体积1µl-20µl皆可），就可以通过荧光酶标仪或荧光仪读取荧光读数（图1）。这项检测方法对常见的污染物如蛋白质、盐类、溶剂、去污剂或游离核苷酸都有很好的耐受性，也适用于在微孔反应板、离心管或比色皿中进行实验。
图 1. 左图：使用荧光酶标仪及iQuant™ microRNA Assay Kit对microRNA进行定量。右图：使用Qubit®荧光仪及iQuant™ microRNA Assay Kit对microRNA进行定量。
技术参数：
| 样品类型： | microRNA |
| 检测平台： | 荧光酶标仪或荧光仪 |
| 线性范围： | 0.5-150 ng microRNA |
应用
microRNA 溶液定量（图2）。
图 2. iQuant™ microRNA 定量检测流程
| Protocol (PDF): | N023 | N024 |
| MSDS (PDF): | MSDS-N023 | MSDS-N024 |
| COA (PDF): | COA-N023 | COA-N024 |
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BMC Med 20, 56 (2022). https://doi.org/10.1186/s12916-022-02254-y
Personalized Postprandial Glucose Response–Targeting Diet Versus Mediterranean Diet for Glycemic Control in Prediabetes
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Diabetes Care 1 September 2021; 44 (9): 1980–1991. https://doi.org/10.2337/dc21-0162
Six genetically linked mutations in the CD36 gene significantly delay the onset of Alzheimer's disease
Sery, O., Zeman, T., Sheardová, K. et al.
Sci Rep 12, 10994 (2022). https://doi.org/10.1038/s41598-022-15299-z