The iQuant™ Protein Assay Kit is designed specifically for an easy and accurate quantitation of protein samples. The kit is highly selective for proteins and is also highly accurate in the presence of reducing reagents, but not in the presence of large amounts of detergent. Common contaminants, such as reducing reagents (e.g., DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, and DNA (but not detergents), are well tolerated in the assay. The kit contains concentrated assay reagent, dilution buffer, and pre-diluted protein standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), then read the concentration using the Qubit™ Fluorometer. The assay is accurate for initial sample concentrations from 12.5 µg/mL to 5 mg/mL and exhibits low protein-to-protein variation. The assay is performed at room temperature, and the signal is stable for 3 hours. The assay can be adapted for use in microplates, tubes or cuvettes.
技术参数：
| 样品类型： | Protein |
| 检测平台: | Fluorescence plate reader or Qubit™ fluorometer |
| 检测范围: | 12.5 µg/mL to 5 mg/mL |
应用
Protein quatitation in solution (Figure 1).
Figure 1. iQuant™ protein assay workflow