The NGS End Repair Module is used to convert 1–5 μg of fragmented DNA to blunt-ended DNA having 5´ phosphates, and 3´-hydroxyls. The module is optimized for use with the NGS dA-Tailing Module (Cat #E113-1, E113-2), and is part of the NGS DNA library prep workflow for Illumina sequencing.
Applications
- End repair of fragmented DNA.
- DNA sample preparation.
Protocol
1. Assemble the following reaction in a microcentrifuge tube on ice:
Fragmented DNA 1-5 µg
End Repair Reaction Buffer (10×) 10 µL
End Repair Enzyme Mix 5 µL
Nuclease-free water to 100 µL
Total volume 100 µL
2. Mix gently and spin down for a few seconds.
3. Incubate at 20°C for 30 min.
4. Purify DNA Sample using AMPure XP beads.
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