NGS End Repair Module

The NGS End Repair Module is used to convert 1–5 μg of fragmented DNA to blunt-ended DNA having 5´ phosphates, and 3´-hydroxyls. The module is optimized for use with the NGS dA-Tailing Module (Cat #E113-1, E113-2), and is part of the NGS DNA library prep workflow for Illumina sequencing.

Applications

• End repair of fragmented DNA.
• DNA sample preparation.

Protocol 

1. Assemble the following reaction in a microcentrifuge tube on ice:

     Fragmented DNA                                           1-5 µg
     End Repair Reaction Buffer (10×)                  10 µL
     End Repair Enzyme Mix                                  5 µL
     Nuclease-free water                                        to 100 µL
    Total volume                                                    100 µL

2. Mix gently and spin down for a few seconds.

3. Incubate at 20°C for 30 min.

4. Purify DNA Sample using AMPure XP beads.