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NGS End Repair Module

Introduction

The NGS End Repair Module is used to convert 1–5 μg of fragmented DNA to blunt-ended DNA having 5´ phosphates, and 3´-hydroxyls. The module is optimized for use with the NGS dA-Tailing Module (Cat #E113-1, E113-2), and is part of the NGS DNA library prep workflow for Illumina sequencing.


Applications

  • End repair of fragmented DNA.
  • DNA sample preparation.


Protocol 

1. Assemble the following reaction in a microcentrifuge tube on ice:

     Fragmented DNA                                           1-5 µg
     End Repair Reaction Buffer (10×)                  10 µL
     End Repair Enzyme Mix                                  5 µL
     Nuclease-free water                                        to 100 µL
    Total volume                                                    100 µL

2. Mix gently and spin down for a few seconds.

3. Incubate at 20°C for 30 min.

4. Purify DNA Sample using AMPure XP beads.

订购

购买 货号 产品名称 规格 价格(¥)
E112-1 NGS End Repair Module  20 rxn 800
E112-2 NGS End Repair Module 100 rxn 3500

   

资料下载

Protocol (PDF): E112