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NGS RNA Second Strand Synthesis Module

Introduction

The NGS Second Strand Synthesis Module contains the enzymes and buffers required to convert first strand cDNA into double stranded cDNA. The fast, user-friendly workflow has minimal hands-on time and is compatible with upstream first strand cDNA synthesis.


Applications

  • RNA-Seq Library Construction.


Protocol 

1.     In a nuclease-free 0.2 mL PCR tube, mix the following components on ice:

         First Strand Synthesis Reaction Buffer            4 μL
         First Strand Synthesis Enzyme Mix                 1 μL
         Nuclease-free Water                                        4 μL
        Total volumes                                                   20 μL

2.     Mix by pipetting gently up and down.

3.     Incubate the samples in a preheated thermal cycler (with the heated lid set to 100°C):

       10 minutes at 25°C
       30 minutes at 68°C
       Hold at 4°C

4.     Place the tube on ice.

5.     Proceed directly to second strand synthesis using NGS RNA Second Strand Synthesis Module (Cat no. E118).

 

订购

购买 货号 产品名称 规格 价格(¥)
E118-1 NGS RNA Second Strand Synthesis Module  25 rxn 2800
E118-2 NGS RNA Second Strand Synthesis Module 100 rxn 9000

   

资料下载

Protocol (PDF): E118