Home » Cell Structure Probes » MitoTrack™ Green FM

 

MitoTrack™ Green FM

Introduction

MitoTrack™ Green FM (Known as MitoTracker® Green FM, Trade Mark of ThermoFisher Scientific) is an green-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is not well-retained after aldehyde fixation. 
 

Specifications:      
Excitation/Emission: 490/516 nm
Shipping Condition: Ambient
Storage Conditions: -20ºC, protect from light
Molecular Formula: C34H28Cl5N3O
Molecular Weight: 671.88
CAS Number: N/A  

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C040 MitoTrack™ Green FM 490/516 1 mg 1200

Documents

 

Protocol (PDF): C040
MSDS (PDF):  MSDS-C040
COA (PDF):  C040

Reference

Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes.
Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X,
Proc Natl Acad Sci U S A (2012) 109:13978-13983

SM-20 is a novel mitochondrial protein that causes caspase-dependent cell death in nerve growth factor-dependent neurons.
Lipscomb EA, Sarmiere PD, Freeman RS
J Biol Chem (2001) 276:5085-5092

Frequently asked questions (FAQs)

It looks like my Mitotracker™ dye is staining more than just the mitochondria. Why?
This is typically a result of using too high of a concentration of the MitoViewr™ dye. Most organic dyes are used in the low micromolar range. The MitoView™ dyes are used at a much lower concentration, around 50–200 nanomolar. Higher concentrations can cause background fluorescence and non-mitochondrial staining.